Journal: Transgenic Research
Article Title: Generation of a Ym1 deficient mouse utilising CRISPR-Cas9 in CB6 embryos
doi: 10.1007/s11248-025-00455-8
Figure Lengend Snippet: To facilitate further characterisation and analysis the CB6 pups carrying the Chil3 deletion were backcrossed onto a BALB/c background. a strategy used to generate CB6 embryos for microinjection. b Speed congenics using the Transnetyx genetic monitoring service was then used to quantify the heterozygosity across each pup’s genome and the animals with the lowest heterozygosity was used for future breeding allowing completed transfer to the BALB/c background in five generations
Article Snippet: Fig. 5 To facilitate further characterisation and analysis the CB6 pups carrying the Chil3 deletion were backcrossed onto a BALB/c background. a strategy used to generate CB6 embryos for microinjection. b Speed congenics using the Transnetyx genetic monitoring service was then used to quantify the heterozygosity across each pup’s genome and the animals with the lowest heterozygosity was used for future breeding allowing completed transfer to the BALB/c background in five generations
Techniques: Microinjection